Show simple item record

dc.contributor.authorGorris, María T.
dc.contributor.authorAlarcon, B.
dc.contributor.authorLópez, María M.
dc.contributor.authorCambra, Mariano
dc.date.accessioned2017-06-01T10:12:09Z
dc.date.available2017-06-01T10:12:09Z
dc.date.issued1994
dc.identifier.citationGorris, M. T., Alarcon, B., Lopez, M.M., Cambra, M. (1994). Characterization of Monoclonal-Antibodies Specific for Erwinia-Carotovora Subsp Atroseptica and Comparison of Serological Methods for its Sensitive Detection on Potato-Tubers. Applied and Environmental Microbiology, 60(6), 2076-2085.
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5329
dc.description.abstractSeven monoclonal antibodies (MAbs) to Erwinia carotovora subsp. atroseptica have been produced. One, called 4G4, reacted with high specificity for serogroup I of E. carotovora subsp. atroseptica, the most common serogroup on potato tubers in different serological assays. Eighty-six strains belonging to different E. carotovora subsp. atroseptica serogroups were assayed. Some strains of serogroup XXII also reacted positively. No cross-reactions were observed against other species of plant pathogenic bacteria or 162 saprophytic bacteria from potato tubers. Only one strain of E. chrysanthemi from potato cross-reacted. A comparison of several serological techniques to detect E. carotovora subsp. atroseptica on potato tubers was performed with MAb 4G4 or polyclonal antibodies. The organism was extracted directly from potato peels of artificially inoculated tubers by soaking or selective enrichment under anaerobiosis in a medium with polypectate. MAb 4G4 was able to detect specifically 240 E. carotovora subsp. atroseptica cells per mi by indirect immunofluorescence and immunofluorescence colony staining and after soaking by ELISA-DAS (double-antibody sandwich enzyme-linked immunosorbent assay) after enrichment. The same amount of cells was detected by using immunolectrotransfer with polyclonal antibodies, and E. carotovora subsp. atroseptica and subsp. carotovora were distinguished by the latter technique. ELISA-DAS using MAb 4G4 with an enrichment step also efficiently detected E. carotovora subsp. atroseptica in naturally infected tubers and plants.
dc.language.isoen
dc.titleCharacterization of Monoclonal-Antibodies Specific for Erwinia-Carotovora Subsp Atroseptica and Comparison of Serological Methods for its Sensitive Detection on Potato-Tubers
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias, Carretera CV-315, Km. 10,7 - 46113 Moncada (València)
dc.date.issuedFreeFormJUN 1994
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.journal.abbreviatedTitleAppl.Environ.Microbiol.
dc.journal.issueNumber6
dc.journal.titleApplied and Environmental Microbiology
dc.journal.volumeNumber60
dc.page.final2085
dc.page.initial2076
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record