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dc.contributor.authorGolmohammadi, M.
dc.contributor.authorLlop, Pablo
dc.contributor.authorScuderi, Giuseppe
dc.contributor.authorGell, I.
dc.contributor.authorGraham, James H.
dc.contributor.authorCubero, Jaime
dc.date.accessioned2017-06-01T10:12:04Z
dc.date.available2017-06-01T10:12:04Z
dc.date.issued2012
dc.identifier.citationGolmohammadi, M., Llop, P., Scuderi, G., Gell, I., Graham, J. H., Cubero, J. (2012). mRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp citri. Plant Pathology, 61(3), 479-488.
dc.identifier.issn0032-0862
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5289
dc.description.abstractThe purpose of this study was to assess the stability of mRNA and rRNA for evaluation of viability for Xanthomonas citri subsp. citri (Xcc). Total RNA from Xcc suspensions subjected to different stress treatments (high temperature or chemical treatment with sodium orthophenylphenate at different concentrations) was extracted at different time periods post-treatment (0, 3, 24 and 48 h) and analysed by quantitative real-time reverse transcription PCR (Q-RT-PCR). Primers were designed from selected fragments of rRNA and mRNA from genes involved in bacterial fitness, virulence or general metabolic mechanisms (gumD, rpfB, avrBs2 and gyrB). After stress treatment, only a 445-bp fragment from the gumD mRNA was detected in live Xcc cells specifically, whereas other RNA fragments, as well as DNA targets, were detected in both viable and nonviable cells. Statistical analyses demonstrated that the amount of some transcripts from genes involved in xanthan synthesis, pathogenicity factor regulation and DNA processing was significantly reduced after lethal treatments. The amplification of the 445-bp product from gumD mRNA was demonstrated to be useful for the detection of viable Xcc; the product was detected specifically from viable bacteria on leaf and citrus fruit surfaces and in citrus canker lesions. Instability of long RNA fragments can be used as a practical tool for the study of survival of citrus canker bacteria or for diagnostic purposes when the presence of viable bacteria needs to be confirmed.
dc.language.isoen
dc.titlemRNA from selected genes is useful for specific detection and quantification of viable Xanthomonas citri subsp citri
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormJUN 2012
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1111/j.1365-3059.2011.02526.x
dc.journal.abbreviatedTitlePlant Pathol.
dc.journal.issueNumber3
dc.journal.titlePlant Pathology
dc.journal.volumeNumber61
dc.page.final488
dc.page.initial479
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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