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dc.contributor.authorGlasa, Miroslav
dc.contributor.authorPredajna, Lukas
dc.contributor.authorSoltys, Katarína
dc.contributor.authorSabanadzovic, Sead
dc.contributor.authorOlmos, Antonio
dc.identifier.citationGlasa, Miroslav, Predajna, Lukas, Soltys, Katarina, Sabanadzovic, Sead, Olmos, A. (2015). Detection and molecular characterisation of Grapevine Syrah virus-1 isolates from Central Europe. Virus genes, 51(1), 112-121.
dc.description.abstractGrapevine Syrah virus-1 (GSyV-1) was identified by small-RNA deep sequencing in Slovak grapevine co-infected by several other viruses. The RT-PCR assays developed in this work substantially improved the virus detection and allowed the identification of GSyV-1 in tested grapevine samples from Slovakia and the Czech Republic at an unexpectedly high rate (ca. 30 %). Subsequently, complete genome sequences of 3 GSyV-1 isolates (2 Slovak and 1 Czech) were determined by Sanger sequencing, showing a typical marafivirus genome organization. Analyses of complete genome sequences showed a higher intra-group diversity among these 3 central European GSyV-1 isolates (differences reaching 7.1 % at the nucleotide level) in comparison to 3 previously characterized North American isolates (only 1.2 % intra-group divergence). A substantially higher divergence among central European isolates and their clustering into two major phylogenetic groups was further confirmed by the partial genome analysis of additional 26 isolates. The CP-centered study did not support the geography-based clustering among central European and American isolates. Nevertheless, the sequence data of the highly variable 5′-proximal portion of the genome obtained for few additional isolates from Slovakia and Czech Republic showed the presence of both, “European-” and “north American-like”, GSyV-1 isolates in the analyzed grapevine samples.
dc.titleDetection and molecular characterisation of Grapevine Syrah virus-1 isolates from Central Europe
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes 2015
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.journal.abbreviatedTitleVirus Genes
dc.journal.titleVirus genes

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