Mostrar el registro sencillo del ítem

dc.contributor.authorAlbiach, M. R.
dc.contributor.authorGuerri, J.
dc.contributor.authorMoreno, P.
dc.date.accessioned2017-06-01T10:11:59Z
dc.date.available2017-06-01T10:11:59Z
dc.date.issued1994
dc.identifier.citationAlbiach, M.R., Guerri, J., Moreno, P. (1994). Multiple-use of Blotted Polyvinylidene Difluoride Membranes Immunostained with Nitro Blue Tetrazolium. Analytical Biochemistry, 221(1), 25-28.
dc.identifier.issn0003-2697
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5254
dc.description.abstractA simple procedure for reusing polyvinylidene difluoride membranes with bound proteins reacted with antibodies and developed by the nitro blue tetrazolium method (M. S. Blake, K. H. Johnston, G. J. Russell-Jones, and E. C. Gotschlich (1984) Anal. Biochem. 136, 175-179) is described. The blue diformazan precipitate produced by the reduction of the nitro blue tetrazolium salt was dissolved by soaking the membrane in dichloro- or trichloromethane for 10-60 min at room temperature. After equilibration in methanol and then in distilled water the first and second antibodies were removed by incubating the membrane in 0.2 M glycine-HCl buffer, pH 2.2, for 90 min at room temperature. These cleaned membranes could be successively reused with different monoclonal antibodies or preimmune serum up to 21 times. The intensity of the spots or electroblotted bands and their specific reaction pattern with different monoclonal antibodies remained unchanged, and no background was observed when the membranes were reacted with preimmune serum. This procedure makes it possible to reuse membranes freshly developed or stored for long periods without loss of resolution. (C) 1994 Academic Press,Inc.
dc.language.isoen
dc.titleMultiple-use of Blotted Polyvinylidene Difluoride Membranes Immunostained with Nitro Blue Tetrazolium
dc.typearticle
dc.date.issuedFreeFormAUG 15 1994
dc.identifier.doi10.1006/abio.1994.1373
dc.journal.abbreviatedTitleAnal.Biochem.
dc.journal.issueNumber1
dc.journal.titleAnalytical Biochemistry
dc.journal.volumeNumber221
dc.page.final28
dc.page.initial25
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


Ficheros en el ítem

FicherosTamañoFormatoVer

No hay ficheros asociados a este ítem.

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem