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dc.contributor.authorGarcia-Mengual, E.
dc.contributor.authorAlfonso, J.
dc.contributor.authorSalvador, I.
dc.contributor.authorDuque, C. -C
dc.contributor.authorSilvestre, M. -A
dc.date.accessioned2017-06-01T10:11:58Z
dc.date.available2017-06-01T10:11:58Z
dc.date.issued2008
dc.identifier.citationGarcia-Mengual, E., Alfonso, J., Salvador, I., Duque, C. -C, Silvestre, M. -A (2008). Oocyte activation procedures and influence of serum on porcine oocyte maturation and subsequent parthenogenetic and nuclear transfer embryo development. Zygote, 16(4), 279-284.
dc.identifier.issn0967-1994
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5250
dc.description.abstractThe viability of SCNT embryos is poor, with an extremely low cloned piglet production rate. In the present work, we studied the effect of three activation protocols based. on ionomycin treatment (5 mu M ionomycin for 5 min and incubated in 2 mM 6-DMAP for 3.5 h) or electric stimuli (two square wave electrical DC pulses of 1.2 kV/cm for 30 mu s) combined or not with 6-DMAP on parthenogenetic embryo development. Oocytes activated by ionomycin plus 6-DMAP showed lower cleavage (47.2 vs. 78.5-81.5; p < 0.05) and blastocyst rates (11.3 vs. 29.2-32.1; p < 0.05) than those activated by electrical and electrical plus 6-DMAP treatments. Also, we studied the effect of addition of serum to maturation medium (0% vs. 10%) on nuclear maturation and further parthenogenetic and SCNT embryo development. We observed in the parthenogenetic embryos that cleavage rates in the serum-free group were significantly higher than in the serum-supplemented group (81.8 vs. 69.6% respectively; p < 0.05), although these differences were not detected in blastocyst rates or blastocyst nuclei numbers. Regarding SCNT embryos, no significant differences were observed in cleavage or blastocyst rates between different experimental groups of SCNT embryos. In conclusion, electrical pulse followed or not by 6-DMAP was found to be an efficient procedure to artificially activate MII porcine oocytes. Moreover, the addition of serum to oocyte maturation media did not seem to improve parthenogenetic or SCNT porcine embryo development.
dc.language.isoen
dc.titleOocyte activation procedures and influence of serum on porcine oocyte maturation and subsequent parthenogenetic and nuclear transfer embryo development
dc.typearticle
dc.date.issuedFreeFormNOV 2008
dc.identifier.doi10.1017/S0967199408004796
dc.journal.abbreviatedTitleZygote
dc.journal.issueNumber4
dc.journal.titleZygote
dc.journal.volumeNumber16
dc.page.final284
dc.page.initial279
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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