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dc.contributor.authorGarcia-Lor, Andres
dc.contributor.authorAncillo, Gema
dc.contributor.authorNavarro, Luis
dc.contributor.authorOllitrault, Patrick
dc.date.accessioned2017-06-01T10:11:57Z
dc.date.available2017-06-01T10:11:57Z
dc.date.issued2013
dc.identifier.citationGarcia-Lor, A., Ancillo, G., Navarro, L., Ollitrault, P. (2013). Citrus (Rutaceae), SNP markers based on Competitive Allele-Specific PCR; transferability across the Aurantioideae subfamily.. Applications in plant sciences, 1(4), -.
dc.identifier.issn2168-0450
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5244
dc.description.abstractUNLABELLED: ; PREMISE OF THE STUDY: Single nucleotide polymorphism (SNP) markers based on Competitive Allele-Specific PCR (KASPar) were developed from sequences of three Citrus species. Their transferability was tested in 63 Citrus genotypes and 19 relative genera of the subfamily Aurantioideae to estimate the potential of SNP markers, selected from a limited intrageneric discovery panel, for ongoing broader diversity analysis at the intra- and intergeneric levels and systematic germplasm bank characterization. ; METHODS AND RESULTS: Forty-two SNP markers were developed using KASPar technology. Forty-one were successfully genotyped in all of the Citrus germplasm, where intra- and interspecific polymorphisms were observed. The transferability and diversity decreased with increasing taxonomic distance. ; CONCLUSIONS: SNP markers based on the KASPar method developed from sequence data of a limited intrageneric discovery panel provide a valuable molecular resource for genetic diversity analysis of germplasm within a genus and should be useful for germplasm fingerprinting at a much broader diversity level.
dc.language.isoen
dc.subjectCompetitive Allele-Specific PCR
dc.subjectRutaceae
dc.subjectgenetic diversity
dc.subjectsingle-nucleotide polymorphisms (SNPs)
dc.titleCitrus (Rutaceae) SNP markers based on Competitive Allele-Specific PCR; transferability across the Aurantioideae subfamily.
dc.typearticle
dc.authorAddressCentro de Proteccion Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, 46113 Moncada (Valencia), Spain.
dc.date.issuedFreeForm2013 Apr
dc.identifier.doi10.3732/apps.1200406
dc.journal.issueNumber4
dc.journal.titleApplications in plant sciences
dc.journal.volumeNumber1
dc.placePublicationUnited States
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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