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dc.contributor.authorGandia, Monica
dc.contributor.authorBernad, Lucia
dc.contributor.authorRubio, Luis
dc.contributor.authorDurán-Vila, Núria
dc.identifier.citationGandia, Monica, Bernad, Lucia, Rubio, L., Duran-Vila, N. (2007). Host effect on the molecular and biological properties of a Citrus exocortis viroid isolate from Vicia faba. Phytopathology, 97(8), 1004-1010.
dc.description.abstractCitrus exocortis viroid (CEVd) is the casual agent of citrus exocortis disease, and has been found in naturally infected citrus and noncitrus hosts. Field isolates of CEVd may infect susceptible hosts as a complex of genetically related sequence variants (haplotypes). In the present work, a CEVd isolate recovered from a symptomless broad bean plant was characterized as a heterogeneous population with a nucleotide diversity of 0.026, which did not contain a predominant haplotype. When nucleic acid extracts of this infected broad bean were used to inoculate tomato, the plants displayed symptoms and the CEVd population was more homogeneous, with a nucleotide diversity of 0.007. However, when nucleic acid extracts from this tomato isolate were back inoculated to new broad bean plants, this isolate did not revert to the original population, because it showed low nucleotide diversity (0.001) and induced symptoms in the broad bean plants. Symptomless broad bean plants may act as reservoirs of highly heterogeneous populations of CEVd variants, providing an excellent inoculum source in terms of its potential to infect a broad range of putative hosts. The epidemiological implications are discussed.
dc.titleHost effect on the molecular and biological properties of a Citrus exocortis viroid isolate from Vicia faba
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes 2007
dc.entidadIVIACentro de Protección Vegetal y Biotecnología

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