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dc.contributor.authorGalipienso, Luis
dc.contributor.authorRubio, Luis
dc.contributor.authorLopez, Carmelo
dc.contributor.authorSoler, Salvador
dc.contributor.authorAramburu, Jose
dc.date.accessioned2017-06-01T10:11:55Z
dc.date.available2017-06-01T10:11:55Z
dc.date.issued2009
dc.identifier.citationGalipienso, L., Rubio, L., Lopez, Carmelo, Soler, Salvador, Aramburu, J. (2009). Complete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato. Virus genes, 39(2), 256-260.
dc.identifier.issn0920-8569
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5228
dc.description.abstractThe genome of a Spanish isolate of Parietaria mottle virus (PMoV) obtained from tomato (strain PMoV-T) was completely sequenced. Protein motifs conserved for RNA viruses were identified: the p1 protein contained a metyltransferase domain in its N-terminal half and a triphosphatase/helicase domain in its C-terminal half, the p2 protein contained a RNA polymerase domain; the 3a protein contained a RNA-binding domain with alpha-helix and beta-sheet secondary structures. In addition, stem-loop structures with potential capacity of protein interactions were predicted on the untranslated terminal regions. Comparison with the other sequenced PMoV isolate showed nucleotide identities of 93, 90, and 93% for genomic RNAs 1, 2 and 3, respectively, and amino acid identities ranging from 88 to 97% for the different proteins. A cytosine deletion was detected at position 1,366 of RNA 3, involving a start codon for the coat protein (CP) gene different from the other PMoV isolate, resulting in a CP 16 amino acids shorter. Comparison of synonymous and nonsynonymous mutations revealed different selective constraints along the genome.
dc.language.isoen
dc.titleComplete nucleotide sequence of a Spanish isolate of Parietaria mottle virus infecting tomato
dc.typearticle
dc.date.issuedFreeFormOCT 2009
dc.identifier.doi10.1007/s11262-009-0388-4
dc.journal.abbreviatedTitleVirus Genes
dc.journal.issueNumber2
dc.journal.titleVirus genes
dc.journal.volumeNumber39
dc.page.final260
dc.page.initial256
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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