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dc.contributor.authorFerrer, Rosa M.
dc.contributor.authorEscriu, F.
dc.contributor.authorLuis-Arteaga, Marisol
dc.contributor.authorGuerri, José
dc.contributor.authorMoreno, Pedro
dc.contributor.authorRubio, Luis
dc.identifier.citationFerrer, R. M., Escriu, F., L.-Arteaga, M., Guerri, J., Moreno, P., Rubio, L. (2008). New molecular methods for identification of Broad bean wilt virus 1. Molecular and cellular probes, 22(4), 223-227.
dc.description.abstractBroad bean wilt virus 1 (BBWV-1) causes damages in economically important plant crops such as pepper, bean, spinach, etc. Fast, cheap and reliable diagnostic tools are crucial to limit or control the disease. In this work, tissue blot immunoassay (TBIA), dot-blot (DB) and tissue-print (TP)-hybridization were developed for BBWV-1 diagnosis and evaluated for sensitivity, specificity and reliability in plants of several host species grown in the greenhouse or in the field, in comparison with ELISA and RT-PCR. RT-PCR followed by DB-hybridization provided the most sensitive and efficient diagnostic, but the virus was also detected in most samples by ELISA. Detection by TBIA or by TP-hybridization avoided sample processing, but they were less consistent and greatly depended on host species and tissue. DB-hybridization with probes corresponding to different genomic regions allowed universal detection of BBWV-1 and discrimination between genetically distant isolates.
dc.titleNew molecular methods for identification of Broad bean wilt virus 1
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes 2008
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.journal.titleMolecular and cellular probes

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