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dc.contributor.authorFadda, Z.
dc.contributor.authorDaros, José A.
dc.contributor.authorFlores, R.
dc.contributor.authorDurán-Vila, Núria
dc.date.accessioned2017-06-01T10:11:50Z
dc.date.available2017-06-01T10:11:50Z
dc.date.issued2003
dc.identifier.citationFadda, Z., Daros, J.A., Flores, R., Duran-Vila, N. (2003). Identification in eggplant of a variant of citrus exocortis viroid (CEVd), with a 96 nucleotide duplication in the right terminal region of the rod-like secondary structure. Virus research, 97(2), 145-149.
dc.identifier.issn0168-1702
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5170
dc.description.abstractAnalysis of eggplants, kept for years under greenhouse conditions after having been mechanically inoculated with nucleic acid preparations from field-grown eggplants containing two viroids, Eggplant latent viroid (ELVd) and Citrus exocortis viroid (CEVd), revealed the presence of an additional larger viroid RNA. Molecular characterization of this RNA showed that it is a stable 467-nt variant of CEVd with a 96-nt duplication of the right terminal region (CEVd-D96) that preserves the rod-like secondary structure. The coexistence in eggplant of CEVd (371 nt) and CEVd-D96, and the fact that they have an almost identical sequence, strongly suggests the emergence of the latter from the former through an internal recombination mediated by a jumping RNA polymerase with low processivity. CEVd-D96 from eggplant is similar to the CEVd-D92 variant characterized previously in a hybrid tomato, suggesting that certain hosts may play a critical role in selecting and replicating this class of enlarged variants.
dc.language.isoen
dc.titleIdentification in eggplant of a variant of citrus exocortis viroid (CEVd) with a 96 nucleotide duplication in the right terminal region of the rod-like secondary structure
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormNOV 2003
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1016/j.virusres.2003.08.002
dc.journal.abbreviatedTitleVirus Res.
dc.journal.issueNumber2
dc.journal.titleVirus research
dc.journal.volumeNumber97
dc.page.final149
dc.page.initial145
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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