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dc.contributor.authorFadda, Z.
dc.contributor.authorDaros, José A.
dc.contributor.authorFagoaga, Carmen
dc.contributor.authorFlores, R.
dc.contributor.authorDurán-Vila, Núria
dc.date.accessioned2017-06-01T10:11:50Z
dc.date.available2017-06-01T10:11:50Z
dc.date.issued2003
dc.identifier.citationFadda, Z., Daros, J.A., Fagoaga, C., Flores, R., Duran-Vila, N. (2003). Eggplant latent viroid, the candidate type species for a new genus within the family Avsunviroidae (hammerhead viroids). Journal of virology, 77(11), 6528-6532.
dc.identifier.issn0022-538X
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5169
dc.description.abstractViroids, small circular RNAs that replicate independently and in most cases incite diseases in plants, are classified into the families Pospiviroidae, composed of species with a central conserved region (CCR) and without hammerhead ribozymes, and Avsunviroidae, composed of three members lacking CCR but able to self-cleave in both polarity strands through hammerhead ribozymes. Here we report the biological and molecular properties of Eggplant latent viroid (ELVd). Purified circular ELVd induces symptomless infections when inoculated into eggplant seedlings. ELVd can be transmitted horizontally and through seed. Sequencing 10 complete cDNA clones showed that ELVd is a circular RNA of 332 to 335 nucleotides with high variability. This RNA can adopt a quasi-rod-like secondary structure of minimal free energy and alternative foldings that permit formation of stable hammerhead structures in plus and minus strands. The ribozymes are active in vitro and, most likely, in vivo. Considering the ELVd properties to be intermediate between those of the two genera of family Avsunviroidae, we propose ELVd as the type species of a third genus with the name Elaviroid.
dc.language.isoen
dc.titleEggplant latent viroid, the candidate type species for a new genus within the family Avsunviroidae (hammerhead viroids)
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormJUN 2003
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1128/JVI.77.11.6528-6532.2003
dc.journal.abbreviatedTitleJ.Virol.
dc.journal.issueNumber11
dc.journal.titleJournal of virology
dc.journal.volumeNumber77
dc.page.final6532
dc.page.initial6528
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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