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dc.contributor.authorDurán-Vila, Núria
dc.contributor.authorGOGORCENA, Y.
dc.contributor.authorORTEGA, V.
dc.contributor.authorORTIZ, J.
dc.contributor.authorNavarro, Luis
dc.date.accessioned2017-06-01T10:11:48Z
dc.date.available2017-06-01T10:11:48Z
dc.date.issued1992
dc.identifier.citationDURANVILA, N., GOGORCENA, Y., ORTEGA, V., ORTIZ, J., Navarro, L. (1992). Morphogenesis and Tissue-Culture of Sweet Orange (Citrus-Sinensis (L), Osb), - Effect of Temperature and Photosynthetic Radiation. Plant Cell Tissue and Organ Culture, 29(1), 11-18.
dc.identifier.issn0167-6857
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5152
dc.description.abstractBoth incubation temperature and photosynthetic radiation affected morphogenesis, callus culture and plantlet culture of sweet orange (Citrus sinensis) cultured in vitro. Bud culture from nodal stem segments, regeneration of shoots and buds from internode stem segments and induction of primary callus were near optimal at incubation temperatures between 21-30-degrees-C. The optimal temperature for root formation was 27-degrees-C with temperatures above and below being clearly deleterious. Incubation in the dark or under low photosynthetic photon flux density (PPFD) was beneficial for callus induction and growth and also favored the production of rooted plantlets from bud cultures. Incubation in the dark improved considerably the regeneration of shoots and buds from internode segments and the recovery of whole plants. No off-types, as determined by protein and isoenzyme analysis, were observed among plantlets recovered from bud cultures or from regeneration of shoots from internode stem segments.
dc.language.isoen
dc.titleMorphogenesis and Tissue-Culture of Sweet Orange (Citrus-Sinensis (L) Osb) - Effect of Temperature and Photosynthetic Radiation
dc.typearticle
dc.date.issuedFreeFormAPR
dc.identifier.doi10.1007/BF00036140
dc.journal.issueNumber1
dc.journal.titlePlant Cell Tissue and Organ Culture
dc.journal.volumeNumber29
dc.page.final18
dc.page.initial11
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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