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dc.contributor.authorDebreczeni, Diana E.
dc.contributor.authorRuiz-Ruiz, Susana
dc.contributor.authorAramburu, Jose
dc.contributor.authorLopez, Carmelo
dc.contributor.authorBelliure, Belén
dc.contributor.authorGalipienso, Luis
dc.contributor.authorSoler, Salvador
dc.contributor.authorRubio, Luis
dc.identifier.citationDebreczeni, D. E., Ruiz-Ruiz, S., Aramburu, J., Lopez, C., Belliure, B., Galipienso, L., Soler, S., Rubio, L. (2011). Detection, discrimination and absolute quantitation of Tomato spotted wilt virus isolates using real time RT-PCR with TaqMan (R), MGB probes. Journal of virological methods, 176(1-2), 32-37.
dc.description.abstractA quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) procedure using a general primer set and three TaqMan (R) MGB probes was developed for general and genotype-specific detection and quantitation of the genomic M segment of Tomato spotted wilt virus (TSWV). Standard curves using RNA transcripts homologous to the three probes allowed reproducible quantitative assays with a wide dynamic range (10(3)-10(10) TSWV M segment RNA copies/ng of total RNA) and high sensitivity. This protocol was assayed with a battery of TSWV isolates, covering the range of the present known genetic variation, in single and/or mix infections in three plant hosts, as well as in the thrips vector Frankliniella occidenialis. This quantitative detection assay will be a valuable tool for molecular biology and epidemiology studies, diagnosis and disease control.
dc.titleDetection, discrimination and absolute quantitation of Tomato spotted wilt virus isolates using real time RT-PCR with TaqMan (R) MGB probes
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes 2011
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.journal.titleJournal of virological methods

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