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dc.contributor.authorCuenca, José
dc.contributor.authorAleza, Pablo
dc.contributor.authorJuárez, José
dc.contributor.authorGarcía-Lor, Andrés
dc.contributor.authorFroelicher, Yann
dc.contributor.authorNavarro, Luis
dc.contributor.authorOllitrault, Patrick
dc.date.accessioned2017-06-01T10:11:39Z
dc.date.available2017-06-01T10:11:39Z
dc.date.issued2015
dc.identifier.citationCuenca, J., Aleza, P., Juarez, J., García-Lor, A., Froelicher, Y., Navarro, L., & Ollitrault, P. (2015). Maximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus. Scientific reports, 5, 9897.
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5069
dc.description.abstractPolyploidisation is a key source of diversification and speciation in plants. Most researchers consider sexual polyploidisation leading to unreduced gamete as its main origin. Unreduced gametes are useful in several crop breeding schemes. Their formation mechanism, i.e., First-Division Restitution (FDR) or Second-Division Restitution (SDR), greatly impacts the gametic and population structures and, therefore, the breeding efficiency. Previous methods to identify the underlying mechanism required the analysis of a large set of markers over large progeny. This work develops a new maximum-likelihood method to identify the unreduced gamete formation mechanism both at the population and individual levels using independent centromeric markers. Knowledge of marker-centromere distances greatly improves the statistical power of the comparison between the SDR and FDR hypotheses. Simulating data demonstrated the importance of selecting markers very close to the centromere to obtain significant conclusions at individual level. This new method was used to identify the meiotic restitution mechanism in nineteen mandarin genotypes used as female parents in triploid citrus breeding. SDR was identified for 85.3% of 543 triploid hybrids and FDR for 0.6%. No significant conclusions were obtained for 14.1% of the hybrids. At population level SDR was the predominant mechanisms for the 19 parental mandarins.
dc.language.isoen
dc.titleMaximum-likelihood method identifies meiotic restitution mechanism from heterozygosity transmission of centromeric loci: application in citrus
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormAPR 20
dc.entidadIVIACentro de Citricultura y Producción Vegetal
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1038/srep09897
dc.journal.titleScientific Reports
dc.journal.volumeNumber5
dc.page.final9897
dc.page.initial9897
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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