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dc.contributor.authorCuenca, José
dc.contributor.authorFroelicher, Y.
dc.contributor.authorAleza, Pablo
dc.contributor.authorJuárez, José
dc.contributor.authorNavarro, Luis
dc.contributor.authorOllitrault, Patrick
dc.date.accessioned2017-06-01T10:11:39Z
dc.date.available2017-06-01T10:11:39Z
dc.date.issued2011
dc.identifier.citationCuenca, J., Froelicher, Y., Aleza, P., Juarez, J., Navarro, L., Ollitrault, P. (2011). Multilocus half-tetrad analysis and centromere mapping in citrus: evidence of SDR mechanism for 2n megagametophyte production and partial chiasma interference in mandarin cv 'Fortune'. Heredity, 107(5), 462-470.
dc.identifier.issn0018-067X
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5067
dc.description.abstractThe genetic structure of 2n gametes and, particularly, the parental heterozygosity restitution at each locus depends on the meiotic process by which they originated, with first-division restitution and second-division restitution (SDR) being the two major mechanisms. The origin of 2n gametes in citrus is still controversial, although sexual polyploidisation is widely used for triploid seedless cultivar development. In this study, we report the analysis of 2n gametes of mandarin cv 'Fortune' by genotyping 171 triploid hybrids with 35 simple sequence repeat markers. The microsatellite DNA allele counting-peak ratios method for allele-dosage evaluation proved highly efficient in segregating triploid progenies and allowed half-tetrad analysis (HTA) by inferring the 2n gamete allelic configuration. All 2n gametes arose from the female genitor. The observed maternal heterozygosity restitution varied between 10 and 82%, depending on the locus, thus SDR appears to be the mechanism underlying 2n gamete production in mandarin cv 'Fortune'. A new method to locate the centromere, based on the best fit between observed heterozygosity restitution within a linkage group and theoretical functions under either partial or no chiasmata interference hypotheses was successfully applied to linkage group II. The maximum value of heterozygosity restitution and the pattern of restitution along this linkage group would suggest there is partial chiasma interference. The implications of such a restitution mechanism for citrus breeding are discussed. Heredity (2011) 107, 462-470; doi:10.1038/hdy.2011.33; published online 18 May 2011
dc.language.isoen
dc.titleMultilocus half-tetrad analysis and centromere mapping in citrus: evidence of SDR mechanism for 2n megagametophyte production and partial chiasma interference in mandarin cv 'Fortune'
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormOCT
dc.entidadIVIACentro de Citricultura y Producción Vegetal
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1038/hdy.2011.33
dc.journal.abbreviatedTitleHeredity
dc.journal.issueNumber5
dc.journal.titleHeredity
dc.journal.volumeNumber107
dc.page.final470
dc.page.initial462
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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