Cyt1Aa protein from Bacillus thuringiensis (Berliner) serovar israelensis is active against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann)

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URI
http://hdl.handle.net/20.500.11939/5052
DOI
10.1002/ps.1965
Derechos de acceso
openAccess
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Author
Cristian Vidal-Quist, J.; Castanera, Pedro; González-Cabrera, Joel
Date
2010
Cita bibliográfica
Cristian Vidal-Quist, J., Castanera, P., Gonzalez-Cabrera, J. (2010). Cyt1Aa protein from Bacillus thuringiensis (Berliner), serovar israelensis is active against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann). Pest management science, 66(9), 949-955.
Abstract
BACKGROUND: Ceratitis capitata (Wiedemann) is one of the world's most destructive fruit pests. The aim of this study was to ascertain insecticidal activity of Bacillus thuringiensis (Berliner) delta-endotoxins to C. capitata. RESULTS: Among 42 selected Bacillus strains, only B. thuringiensis serovar israelensis (Bti) solubilised protoxins showed biological activity against C. capitata neonate larvae, whereas Bti spore and crystal mixture was inactive. Insecticidal activity of Bti protoxins was significantly enhanced by incubation with Culex pipiens L. gut extracts. Overdigestion of Bti protoxins with Sesamia nonagrioides (Lefebvre) gut extracts suppressed biological activity against C. capitata, and this correlated with degradation of Cyt toxins. Cyt1Aa solubilised protoxin showed the highest toxicity, LC50 after 7 days of 4.93 mu g cm(-2), while proteolytical processing of Cyt1Aa protoxins by larval gut extracts did not enhance insecticidal activity. CONCLUSION: The present study provides evidence for the first time of the insecticidal activity of a B. thuringiensis strain against C. capitata and identifies a single delta-endotoxin with potential for controlling this pest. (C) 2010 Society of Chemical Industry
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