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dc.contributor.authorCohn, Marianne T.
dc.contributor.authorKjelgaard, Peter
dc.contributor.authorFrees, Dorte
dc.contributor.authorPenadés, José R.
dc.contributor.authorIngmer, Hanne
dc.date.accessioned2017-06-01T10:11:34Z
dc.date.available2017-06-01T10:11:34Z
dc.date.issued2011
dc.identifier.citationCohn, M.nne T., Kjelgaard, Peter, Frees, Dorte, Penades, J.R., Ingmer, Hanne (2011). Clp-dependent proteolysis of the LexA N-terminal domain in Staphylococcus aureus. Microbiology-Sgm, 157, 677-684.
dc.identifier.issn1350-0872
dc.identifier.urihttp://hdl.handle.net/20.500.11939/5033
dc.description.abstractThe SOS response is governed by the transcriptional regulator LexA and is elicited in many bacterial species in response to DNA damaging conditions. Induction of the SOS response is mediated by autocleavage of the LexA repressor resulting in a C-terminal dimerization domain (CTD) and an N-terminal DNA-binding domain (NTD) known to retain some DNA-binding activity. The proteases responsible for degrading the LexA domains have been identified in Escherichia coli as ClpXP and Lon. Here, we show that in the human and animal pathogen Staphylococcus aureus, the ClpXP and ClpCP proteases contribute to degradation of the NTD and to a lesser degree the CTD. In the absence of the proteolytic subunit, ClpP, or one or both of the Clp ATPases, ClpX and ClpC, the LexA domains were stabilized after autocleavage. Production of a stabilized variant of the NTD interfered with mitomycin-mediated induction of sosA expression while leaving lexA unaffected, and also significantly reduced SOS-induced mutagenesis. Our results show that sequential proteolysis of LexA is conserved in S. aureus and that the NTD may differentially regulate a subset of genes in the SOS regulon.
dc.language.isoen
dc.titleClp-dependent proteolysis of the LexA N-terminal domain in Staphylococcus aureus
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormMAR 2011
dc.entidadIVIACentro de Tecnología Animal
dc.identifier.doi10.1099/mic.0.043794-0
dc.journal.titleMicrobiology-Sgm
dc.journal.volumeNumber157
dc.page.final684
dc.page.initial677
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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