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dc.contributor.authorCecilia Zanek, Maria
dc.contributor.authorAndrea Reyes, Carina
dc.contributor.authorCervera, Magdalena
dc.contributor.authorPena, Eduardo Jose
dc.contributor.authorVelázquez, Karelia
dc.contributor.authorCosta, Norma
dc.contributor.authorInes Plata, Maria
dc.contributor.authorGrau, Oscar
dc.contributor.authorPena, Leandro
dc.contributor.authorLaura Garcia, Maria
dc.identifier.citationCecilia Zanek, M., Andrea Reyes, Carina, Cervera, M., J. Pena, E., Velazquez, Karelia, Costa, Norma, Ines Plata, M., Grau, O., Pena, L., L. Garcia, M. (2008). Genetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus. Plant Cell Reports, 27(1), 57-66.
dc.description.abstractCitrus psorosis is a serious viral disease affecting citrus trees in many countries. Its causal agent is Citrus psorosis virus (CPsV), the type member of genus Ophiovirus. CPsV infects most important citrus varieties, including oranges, mandarins and grapefruits, as well as hybrids and citrus relatives used as rootstocks. Certification programs have not been sufficient to control the disease and no sources of natural resistance have been found. Pathogen-derived resistance (PDR) can provide an efficient alternative to control viral diseases in their hosts. For this purpose, we have produced 21 independent lines of sweet orange expressing the coat protein gene of CPsV and five of them were challenged with the homologous CPV 4 isolate. Two different viral loads were evaluated to challenge the transgenic plants, but so far, no resistance or tolerance has been found in any line after 1 year of observations. In contrast, after inoculation all lines showed characteristic symptoms of psorosis in the greenhouse. The transgenic lines expressed low and variable amounts of the cp gene and no correlation was found between copy number and transgene expression. One line contained three copies of the cp gene, expressed low amounts of the mRNA and no coat protein. The ORF was cytosine methylated suggesting a PTGS mechanism, although the transformant failed to protect against the viral load used. Possible causes for the failed protection against the CPsV are discussed.
dc.titleGenetic transformation of sweet orange with the coat protein gene of Citrus psorosis virus and evaluation of resistance against the virus
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes 2008
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.journal.abbreviatedTitlePlant Cell Rep.
dc.journal.titlePlant Cell Reports

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