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dc.contributor.authorCallejas, C.
dc.contributor.authorVelasco, A.
dc.contributor.authorGobbi, A.
dc.contributor.authorBeitia, Francisco J.
dc.contributor.authorOchando, M. D.
dc.date.accessioned2017-06-01T10:11:22Z
dc.date.available2017-06-01T10:11:22Z
dc.date.issued2005
dc.identifier.citationCallejas, C., Velasco, A., Gobbi, A., Beitia, F., Ochando, M. D. (2005). Fast discrimination (RAPD-PCR), of the species forming the pest complex Aleurodicus dispersus-Lecanoideus floccissimus (Hom : Aleyrodidae). Journal of Applied Entomology, 129(7), 382-385.
dc.identifier.issn0931-2048
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4930
dc.description.abstractThe Aleurodicus dispersus-Lecanoideus floccissimus complex has become a very important agricultural pest in the Canaries. These species are not easily differentiated by their morphological characteristics. The aim of the present study was to obtain genetic markers to unambiguously distinguish both species of this complex. Thus, six random primers were employed to generate RAPD markers. Different RAPD profiles were observed for the different species. The analysis successfully identified 39 reproducible and specific markers for L. floccissimus and 51 for A. dispersus, i.e. bands present in all individuals of one species but never in the other. RAPD markers resulted a useful tool for discriminate both species. Early identification of species and also of biotypes is crucial when designing control strategies to avoid the spread of the pest and consequently the considerable economic losses it causes in tropical crops.
dc.language.isoen
dc.titleFast discrimination (RAPD-PCR) of the species forming the pest complex Aleurodicus dispersus-Lecanoideus floccissimus (Hom : Aleyrodidae)
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada, España.
dc.date.issuedFreeFormAUG 2005
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1111/j.1439-0418.2005.00982.382-385
dc.journal.abbreviatedTitleJ.Appl.Entomol.
dc.journal.issueNumber7
dc.journal.titleJournal of Applied Entomology
dc.journal.volumeNumber129
dc.page.final385
dc.page.initial382
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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