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dc.contributor.authorAgüero, Jesús
dc.contributor.authorVives, María C.
dc.contributor.authorVelázquez, Karelia
dc.contributor.authorRuiz-Ruiz, Susana
dc.contributor.authorJuárez, José
dc.contributor.authorNavarro, Luis
dc.contributor.authorMoreno, Pedro
dc.contributor.authorGuerri, José
dc.date.accessioned2017-06-01T10:11:19Z
dc.date.available2017-06-01T10:11:19Z
dc.date.issued2013
dc.identifier.citationAguero, Jesus, Vives, M.C., Velazquez, Karelia, Ruiz-Ruiz, S., Juarez, J., Navarro, L., Moreno, P., Guerri, J. (2013). Citrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus.. Molecular plant pathology, 14(6), 610-6.
dc.identifier.issn1364-3703
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4910
dc.description.abstractTo invade systemically host plants, viruses need to replicate in the infected cells, spread to neighbouring cells through plasmodesmata and move to distal parts of the plant via sieve tubes to start new infection foci. To monitor the infection of Nicotiana benthamiana plants by Citrus leaf blotch virus (CLBV), leaves were agroinoculated with an infectious cDNA clone of the CLBV genomic RNA expressing green fluorescent protein (GFP) under the transcriptional control of a duplicate promoter of the coat protein subgenomic RNA. Fluorescent spots first appeared in agroinfiltrated leaves 11-12 days after infiltration, indicating CLBV replication. Then, after entering the phloem vascular system, CLBV was unloaded in the upper parts of the plant and invaded all tissues, including flower organs and meristems. GFP fluorescence was not visible in citrus plants infected with CLBV-GFP. Therefore, to detect CLBV in meristematic regions, Mexican lime (Citrus aurantifolia) plants were graft inoculated with CLBV, with Citrus tristeza virus (CTV), a virus readily eliminated by shoot-tip grafting invitro, or with both simultaneously. Although CLBV was detected by hybridization and real-time reverse transcription-polymerase chain reaction (RT-PCR) in 0.2-mm shoot tips in all CLBV-inoculated plants, CTV was not detected. These results explain the difficulty in eliminating CLBV by shoot-tip grafting invitro.
dc.language.isoen
dc.titleCitrus leaf blotch virus invades meristematic regions in Nicotiana benthamiana and citrus.
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeForm2013-Aug
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1111/mpp.12031
dc.journal.issueNumber6
dc.journal.titleMolecular plant pathology
dc.journal.volumeNumber14
dc.page.final6
dc.page.initial610
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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