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dc.contributor.authorAgüero, Jesús
dc.contributor.authorRuiz-Ruiz, Susana
dc.contributor.authorVives, María C.
dc.contributor.authorVelázquez, Karelia
dc.contributor.authorNavarro, Luis
dc.contributor.authorPena, Leandro
dc.contributor.authorMoreno, Pedro
dc.contributor.authorGuerri, José
dc.date.accessioned2017-06-01T10:11:14Z
dc.date.available2017-06-01T10:11:14Z
dc.date.issued2012
dc.identifier.citationAgueero, Jesus, Ruiz-Ruiz, S., Vives, M.C., Velazquez, Karelia, Navarro, L., Pena, L., Moreno, P., Guerri, J. (2012). Development of Viral Vectors Based on Citrus leaf blotch virus to Express Foreign Proteins or Analyze Gene Function in Citrus Plants. Molecular Plant-Microbe Interactions, 25(10), 1326-1337.
dc.identifier.issn0894-0282
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4877
dc.description.abstractViral vectors have been used to express foreign proteins in plants or to silence endogenous genes. This methodology could be appropriate for citrus plants that have long juvenile periods and adult plants that are difficult to transform. We developed viral vectors based on Citrus leaf blotch virus (CLBV) by duplicating a minimum promoter (92 bp) either at the 3′ untranslated region (clbv3′pr vector) or at the intergenic region between the movement and coat protein (CP) genes (clbvINpr vector). The duplicated fragment (–42/+50) around the transcription start site of the CP subgenomic RNA (sgRNA) had the full promoter activity and induced synthesis of a new sgRNA in infected plants. Agroinoculation with these vectors resulted in systemic infection of Nicotiana benthamiana and the resulting virions systemically infected citrus plants. A clbvINpr vector carrying the green fluorescent protein (GFP) gene expressed GFP in citrus plants and triggered gfp silencing in gfp-transgenic citrus plants, and vectors carrying fragments of the phytoene desaturase or the magnesium chelatase genes incited a silencing phenotype in citrus plants. These silenced phenotypes persisted in successive flushes. Because CLBV infections are symptomless in most citrus species, the effective silencing induced by CLBV-derived vectors will be helpful to analyze citrus gene function.
dc.language.isoen
dc.titleDevelopment of Viral Vectors Based on Citrus leaf blotch virus to Express Foreign Proteins or Analyze Gene Function in Citrus Plants
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormOCT 2012
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1094/MPMI-02-12-0048-R
dc.journal.abbreviatedTitleMol.Plant-Microbe Interact.
dc.journal.issueNumber10
dc.journal.titleMolecular Plant-Microbe Interactions
dc.journal.volumeNumber25
dc.page.final1337
dc.page.initial1326
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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