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dc.contributor.authorBertolini, Edson
dc.contributor.authorTeresani, G. R.
dc.contributor.authorLoiseau, M.
dc.contributor.authorTanaka, F. A. O.
dc.contributor.authorBarbé, Silvia
dc.contributor.authorMartinez, C.
dc.contributor.authorGentit, P.
dc.contributor.authorLópez, María M.
dc.contributor.authorCambra, Mariano
dc.date.accessioned2017-06-01T10:11:08Z
dc.date.available2017-06-01T10:11:08Z
dc.date.issued2015
dc.identifier.citationBertolini, E., Teresani, G. R., Loiseau, M., Tanaka, F. A. O., Barbe, S., Martinez, C., Gentit, P., Lopez, M.M., Cambra, M. (2015). Transmission of 'Candidatus Liberibacter solanacearum' in carrot seeds. Plant Pathology, 64(2), 276-285.
dc.identifier.issn0032-0862
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4838
dc.description.abstractA protocol for the specific detection and quantification of Candidatus Liberibacter solanacearum' in carrot seeds using real-time PCR was developed. The bacterium was detected in 23 out of 54 carrot seed lots from 2010 to 2014, including seeds collected from diseased mother plants. The average total number of Ca. L. solanacearum' cells in individual seeds ranged from 48 +/- 33 to 210 +/- 67cells per seed from three seed lots, but using propidium monoazide to target live cells, 95% of the cells in one seed lot were found to be dead. Liberibacter-like cells were observed in the phloem sieve tubes of the seed coat and in the phloem of carrot leaf midrib from seedlings. The bacterium was detected as early as 30days post-germination, but more consistently after 90days, in seedlings grown from PCR positive seed lots in an insect-proof P2 level containment greenhouse. Between 12% and 42% of the seedlings from positive seed lots tested positive for Ca. L. solanacearum'. After 150days, symptoms of proliferation were observed in 12% of seedlings of cv. Maestro. Candidatus Liberibacter solanacearum' haplotype E was identified in the seeds and seedlings of cv. Maestro. No phytoplasmas were detected in seedlings with symptoms using a real-time assay for universal detection of phytoplasmas. The results show that to prevent the entry and establishment of the bacterium in new areas and its potential spread to other crops, control of Ca. L. solanacearum' in seed lots is required.
dc.language.isoen
dc.subjectcell viability
dc.subjectdetection
dc.subjectelectron microscopy
dc.subjectquantification
dc.subjectreal-time PCR
dc.subjectseedborne bacterium
dc.subjectZEBRA CHIP DISEASE
dc.subject1ST REPORT
dc.subjectELECTRON-MICROSCOPY
dc.subjectUNITED-STATES
dc.subjectCITRUS SEED
dc.subjectPOTATO
dc.subjectASSOCIATION
dc.subjectASIATICUS
dc.subjectMEXICO
dc.titleTransmission of 'Candidatus Liberibacter solanacearum' in carrot seeds
dc.typearticle
dc.date.issuedFreeFormAPR
dc.identifier.doi10.1111/ppa.12245
dc.journal.abbreviatedTitlePlant Pathol.
dc.journal.issueNumber2
dc.journal.titlePlant Pathology
dc.journal.volumeNumber64
dc.page.final285
dc.page.initial276
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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