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dc.contributor.authorBertolini, Edson
dc.contributor.authorPenyalver, Ramón
dc.contributor.authorGarcia, Amparo
dc.contributor.authorOlmos, Antonio
dc.contributor.authorQuesada, Jose M.
dc.contributor.authorCambra, Mariano
dc.contributor.authorLópez, María M.
dc.date.accessioned2017-06-01T10:11:08Z
dc.date.available2017-06-01T10:11:08Z
dc.date.issued2003
dc.identifier.citationBertolini, E., Penyalver, R., Garcia, A., Olmos, A., Quesada, J.M., Cambra, M., Lopez, M.M. (2003). Highly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube. Journal of microbiological methods, 52(2), 261-266.
dc.identifier.issn0167-7012
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4837
dc.description.abstractA nested-polymerase chain reaction (PCR) has been set up to be performed in a single closed tube for the detection of Pseudomonas savastanoi pv. savastanoi. Nested-PCR coupled with dot-blot hybridization was able to detect up to one cell of the target per ml of olive extract, showing the greatest sensitivity compared with all previously reported detection assays. Validation of the developed procedure for diagnosis and epidemiological purposes was achieved by testing ca. 240 asymptomatic plant samples from olive trees. When performing the other previously reported techniques (bacterial isolation and single PCR), P. savastanoi was detected in 50 of the analyzed samples, while with the new developed nested-PCR assay, the bacterium was detected in 82 samples.
dc.language.isoen
dc.titleHighly sensitive detection of Pseudomonas savastanoi pv. savastanoi in asymptomatic olive plants by nested-PCR in a single closed tube
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormFEB 2003
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1016/S0167-7012(02)00163-X
dc.journal.abbreviatedTitleJ.Microbiol.Methods
dc.journal.issueNumber2
dc.journal.titleJournal of microbiological methods
dc.journal.volumeNumber52
dc.page.final266
dc.page.initial261
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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