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dc.contributor.authorZagrai, Luminita Antonela
dc.contributor.authorCapote, Nieves
dc.contributor.authorRavelonandro, M.
dc.contributor.authorCambra, Mariano
dc.contributor.authorZagrai, L.
dc.contributor.authorScorza, Ralph
dc.date.accessioned2017-06-01T10:10:58Z
dc.date.available2017-06-01T10:10:58Z
dc.date.issued2008
dc.identifier.citationZagrai, I., Capote, N., Ravelonandro, M., Cambra, M., Zagrai, L., Scorza, R. (2008). Plum pox virus silencing of C5 transgenic plums is stable under challenge inoculation with heterologous viruses. Journal of Plant Pathology, 90(1), 63-71.
dc.identifier.issn1125-4653
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4775
dc.description.abstractTransgenic C5 cv. Honey Sweet is a clone of Prunus domestica L. transformed with the Plum pox virus coat protein gene (PPV-CP). This transgenic plum displays post-transcriptional gene silencing (PTGS) which makes it highly resistant to PPV infection. To test the effect of heterologous viruses on the efficacy and stability of PTGS against PPV, transgenic C5 trees were graft-inoculated with different combinations of Prunus necrotic ringspot virus (PNRSV), Apple chlorotic leaf spot virus (ACLSV), Prune dwarf virus (PDV) and PPV-D strain. The potential for suppression of the silencing mechanism mediated by these viruses was evaluated. Challenge experiments were performed under greenhouse, nursery and field conditions in Romania and Spain, comprising two different environments, continental and Mediterranean, respectively. Virus infections were evaluated by visual monitoring of symptom and by serological and molecular assays. The engineered resistance to PPV in C5 transgenic plums was stable and was not suppressed by the presence of the challenging heterologous viruses over a three-year experimental period across all trials.
dc.language.isoen
dc.titlePlum pox virus silencing of C5 transgenic plums is stable under challenge inoculation with heterologous viruses
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormMAY 2008
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.journal.abbreviatedTitleJ.Plant Pathol.
dc.journal.issueNumber1
dc.journal.titleJournal of Plant Pathology
dc.journal.volumeNumber90
dc.page.final71
dc.page.initial63
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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