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dc.contributor.authorVives, María C.
dc.contributor.authorRubio, Luis
dc.contributor.authorGalipienso, Luis
dc.contributor.authorNavarro, Luis
dc.contributor.authorMoreno, Pedro
dc.contributor.authorGuerri, José
dc.date.accessioned2017-06-01T10:10:57Z
dc.date.available2017-06-01T10:10:57Z
dc.date.issued2002
dc.identifier.citationVives, MC, Rubio, L., Galipienso, L., Navarro, L., Moreno, P., Guerri, J. (2002). Low genetic variation between isolates of Citrus leaf blotch virus from different host species and of different geographical origins. Journal of General Virology, 83, 2587-2591.
dc.identifier.issn0022-1317
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4770
dc.description.abstractThe population structure and genetic diversity of Citrus leaf blotch virus (CLBV) were estimated by single-strand conformation polymorphism and nucleotide sequence analyses of two genomic regions located within the replicase (R) and the coat protein (C) genes. Analysis of 30 cDNA clones of each genomic region from two CLBV isolates showed that both isolates contained a predominant haplotype and others closely related. Analysis of 37 CLBV Spanish field isolates showed low genetic diversity (0.0041 and 0.0018 for genomic regions R and C, respectively). Comparison of 14 CLBV isolates from Spain, Japan, USA, France and Australia showed genetic diversities of 0.0318 (R) and 0.0209 (C), respectively. No correlation was found between genetic distance and geographical origin or host species of the isolates. The ratio between nonsynonymous and synonymous substitutions was the lowest found in a plant virus, indicating a strong negative selective pressure in both genomic regions.
dc.language.isoen
dc.titleLow genetic variation between isolates of Citrus leaf blotch virus from different host species and of different geographical origins
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormOCT
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1099/0022-1317-83-10-2587
dc.journal.abbreviatedTitleJ.Gen.Virol.
dc.journal.titleJournal of General Virology
dc.journal.volumeNumber83
dc.page.final2591
dc.page.initial2587
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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