Show simple item record

dc.contributor.authorVives, María C.
dc.contributor.authorMartin, Susana
dc.contributor.authorAmbrós, Silvia
dc.contributor.authorRenovell, Agueda
dc.contributor.authorNavarro, Luis
dc.contributor.authorPina, José A.
dc.contributor.authorMoreno, Pedro
dc.contributor.authorGuerri, José
dc.date.accessioned2017-06-01T10:10:57Z
dc.date.available2017-06-01T10:10:57Z
dc.date.issued2008
dc.identifier.citationVives, M.C., Martin, S., Ambros, S., Renovell, Agueda, Navarro, L., Pina, J.A., Moreno, P., Guerri, J. (2008). Development of a full-genome cDNA clone of Citrus leaf blotch virus and infection of citrus plants. Molecular Plant Pathology, 9(6), 787-797.
dc.identifier.issn1464-6722
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4767
dc.description.abstractCitrus leaf blotch virus (CLBV), a member of the family Flexiviridae, has a similar to 9-kb single-stranded, positive-sense genomic RNA encapsidated by a 41-kDa coat protein. CLBV isolates are associated with symptom production in citrus including leaf blotching of Dweet tangor and stem pitting in Etrog citron (Dweet mottle disease), and some isolates are associated with bud union crease on trifoliate rootstocks, but Koch's postulates for this virus were not fulfilled. A full-genome cDNA of CLBV isolate SRA-153, which induces bud union crease, was placed under the T7 promoter (clone T7-CLBV), or between the 35S promoter and the Nos-t terminator, with or without a ribozyme sequence downstream of the CLBV sequence (clones 35SRbz-CLBV and 35S-CLBV). RNA transcripts from T7-CLBV failed to infect Etrog citron and Nicotiana occidentalis and N. benthamiana plants, whereas agro-inoculation with binary vectors carrying 35SRbz-CLBV or 35S-CLBV, and the p19 silencing suppressor, caused systemic infection and production of normal CLBV virions. Virus accumulation was similar in citron plants directly agro-infiltrated, or mechanically inoculated with wild-type or 35SRbz-CLBV-derived virions from Nicotiana, and the three sources incited the symptoms characteristic of Dweet mottle disease, but not bud union crease. Our results show that (1) virions derived from an infectious clone show the same replication, movement and pathogenicity characteristics as the wild-type CLBV; (2) CLBV is the causal agent of Dweet mottle disease but not of the bud union crease syndrome; and (3) for the first time an RNA virus could be successfully agro-inoculated on citrus plants. This infectious clone may become a useful viral vector for citrus genomic studies.
dc.language.isoen
dc.titleDevelopment of a full-genome cDNA clone of Citrus leaf blotch virus and infection of citrus plants
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormNOV
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1111/J.1364-3703.2008.00501.X
dc.journal.issueNumber6
dc.journal.titleMolecular Plant Pathology
dc.journal.volumeNumber9
dc.page.final797
dc.page.initial787
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


Files in this item

This item appears in the following Collection(s)

Show simple item record