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dc.contributor.authorVidal, E.
dc.contributor.authorMoreno, A.
dc.contributor.authorBertolini, Edson
dc.contributor.authorCambra, M.
dc.date.accessioned2017-06-01T10:10:50Z
dc.date.available2017-06-01T10:10:50Z
dc.date.issued2012
dc.identifier.citationVidal, E., Moreno, A., Bertolini, E., Cambra, M. (2012). Estimation of the accuracy of two diagnostic methods for the detection of Plum pox virus in nursery blocks by latent class models. Plant Pathology, 61(2), 413-422.
dc.identifier.issn0032-0862
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4719
dc.description.abstractThe control of Plum pox virus (PPV), the most important viral disease that affects stone fruit trees, requires the use of reliable detection methods. The effectiveness of spot real-time reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of PPV in samples collected from nursery blocks was compared with a validated PPV detection technique, the double antibody sandwich indirect enzyme-linked immunosorbent assay (DASI-ELISA) using the PPV-specific monoclonal antibody 5B-IVIA/AMR. In total, 5047 nursery plants were analysed by both techniques. The agreement between the techniques was almost perfect (Cohens kappa index of 0.88 +/- 0.01). The diagnostic parameters (sensitivity, specificity and likelihood ratios) of both techniques were simultaneously evaluated in 2473 nursery plants by latent class models using maximum likelihood functions and a Bayesian approach. The sensitivity and specificity of both techniques did not vary according to the latent model applied. Spot real-time RT-PCR was more sensitive while DASI-ELISA was more specific for PPV detection. In addition, the findings demonstrate that latent class models are a flexible and potent statistical method to estimate the accuracy of diagnostic tests for plant pathology.
dc.language.isoen
dc.titleEstimation of the accuracy of two diagnostic methods for the detection of Plum pox virus in nursery blocks by latent class models
dc.typearticle
dc.date.issuedFreeFormAPR 2012
dc.identifier.doi10.1111/j.1365-3059.2011.02505.x
dc.journal.abbreviatedTitlePlant Pathol.
dc.journal.issueNumber2
dc.journal.titlePlant Pathology
dc.journal.volumeNumber61
dc.page.final422
dc.page.initial413
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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