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dc.contributor.authorTorres, E.
dc.contributor.authorBertolini, Edson
dc.contributor.authorCambra, Mariano
dc.contributor.authorMonton, C.
dc.contributor.authorMartin, M. P.
dc.date.accessioned2017-06-01T10:10:32Z
dc.date.available2017-06-01T10:10:32Z
dc.date.issued2005
dc.identifier.citationTorres, E., Bertolini, E., Cambra, M., Monton, C., Martin, M.P. (2005). Real-time PCR for simultaneous and quantitative detection of quarantine phytoplasmas from apple proliferation (16SrX), group. Molecular and cellular probes, 19(5), 334-340.
dc.identifier.issn0890-8508
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4609
dc.description.abstractA real time PCR assay conjugated with the fluorescent SYBR(R) Green I dye has been developed for rapid, sensitive and quantitative ;detection of 'Ca. Phytoplasma pyri', 'Co. P. prunorum' and 'Ca. P. mali', quarantine members of apple proliferation (16SrX) group. The selected primers amplify specifically a target of 217-bp fragment from the 16Sr gene region of the 16SrX group and not from any other tested phytoplasma groups. An artificial template consisting in a plasmid clone of a 1785-bp DNA fragment of the 16S rRNA gene, 16S/23S rDNA spacer region, tRNA-Ile and partial 23S rRNA gene of a 'Ca. P. prunorum' isolate, was used to establish a calibration curve to evaluate the number of amplified targets per sample. The sensitivity of the technique was similar to nested-PCR (10 copies of the amplified target per mu l). The estimated concentration of phytoplasmas in infected pear, plum and apricot trees ranged from 9.7 X 10(3) to 3.0 X 10(5) phytoplasmas per gram of tissue. The method offers the possibility to detect simultaneously, in a single reaction, all quarantine phytoplasmas affecting fruit trees hosts in Europe. (C) 2005 Elsevier Ltd. All rights reserved.
dc.language.isoen
dc.titleReal-time PCR for simultaneous and quantitative detection of quarantine phytoplasmas from apple proliferation (16SrX) group
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias, Carretera CV-315, Km. 10,7 - 46113 Moncada (València)
dc.date.issuedFreeFormOCT 2005
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1016/j.mcp.2005.06.002
dc.journal.abbreviatedTitleMol.Cell.Probes
dc.journal.issueNumber5
dc.journal.titleMolecular and cellular probes
dc.journal.volumeNumber19
dc.page.final340
dc.page.initial334
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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