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dc.contributor.authorTomas, Cristina
dc.contributor.authorBlanch, Eva
dc.contributor.authorHernandez, Marta
dc.contributor.authorGil, Maria A.
dc.contributor.authorRoca, Jordi
dc.contributor.authorVazquez, Juan M.
dc.contributor.authorMartinez, Emilio A.
dc.contributor.authorMoce, Eva
dc.date.accessioned2017-06-01T10:10:30Z
dc.date.available2017-06-01T10:10:30Z
dc.date.issued2011
dc.identifier.citationTomas, C., Blanch, Eva, Hernandez, Marta, Gil, M.A., Roca, Jordi, Vazquez, J. M., Martinez, Emilio A., Moce, E. (2011). Treating boar sperm with cholE.ol-loaded cyclodextrins widens the sperm osmotic tolerance limits and enhances the in vitro sperm fertilising ability. Animal Reproduction Science, 129(3-4), 209-220.
dc.identifier.issn0378-4320
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4593
dc.description.abstractTreating sperm with cholesterol-loaded cyclodextrins (CLC) improves the cryosurvival of the sperm of different cold-shock sensitive species. However, the response of boar sperm to this treatment is not fully understood. The aim of this study was to determine how CLC and methyl-beta-cyclodextrin (M beta CD, not loaded with cholesterol) affect the parameters for boar sperm functionality, including sperm osmotic resistance, and the ability of the sperm to capacitate and to penetrate the sow's immature oocytes in vitro. Samples treated with CLC or M beta CD prior to freezing exhibited similar percentages of motile sperm, live sperm and sperm with intact acrosomes as the control samples (P > 0.05). In addition, these treatments did not alter the response of the boar sperm to capacitating conditions. However, when compared to the controls and the M beta CD-treated samples, the CLC-treated sperm maintained greater percentages of motile sperm and live sperm in a wide range of osmotic solutions including hypo- (50.75 and 150 mOsm/kg) and hyper-osmotic (600, 800 mOsm/kg) conditions (P < 0.05). In addition, the CLC-treated sperm exhibited greater oocyte penetration ability than the control and the M beta CD-treated sperm (P < 0.0001). In conclusion, the pre-freezing treatment of boar sperm with CLC does not alter the ability of the sperm to respond to capacitating conditions. Despite not increasing the cryosurvival of the sperm, this treatment widens the sperm osmotic tolerance limits and enhances the in vitro sperm fertilising ability. (C) 2011 Elsevier B.V. All rights reserved.
dc.language.isoen
dc.titleTreating boar sperm with cholesterol-loaded cyclodextrins widens the sperm osmotic tolerance limits and enhances the in vitro sperm fertilising ability
dc.typearticle
dc.date.issuedFreeFormDEC 2011
dc.identifier.doi10.1016/j.anireprosci.2011.12.003
dc.journal.abbreviatedTitleAnim.Reprod.Sci.
dc.journal.issueNumber3-4
dc.journal.titleAnimal Reproduction Science
dc.journal.volumeNumber129
dc.page.final220
dc.page.initial209
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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