Addition of Cholesterol-Loaded Cyclodextrins to the Thawing Extender: Effects on Boar Sperm Quality
Derechos de accesoopenAccess
MetadatosMostrar el registro completo del ítem
Contents The aim of the present study was to evaluate the effect that the addition of cholesterol-loaded cyclodextrins (CLC) to the thawing extender has on the quality of frozen-thawed boar sperm. Pooled semen (n=5) from three boars was used for the experiments. The semen was cryopreserved with an egg-yolk-based extender, it was diluted after thawing in Beltsville thawing solution (BTS) supplemented with different concentrations of CLC (0, 12.5, 25, 50 or 100mg/500x10(6) sperm), and these samples were incubated at 37 degrees C for 150min. The following parameters of sperm quality were evaluated 30 and 150min after incubation: sperm with intact plasma membrane (SIPM; %), sperm with normal acrosomal ridge (NAR; %), total motile sperm (TMS; %), progressively motile sperm (PMS; %) and kinetic parameters. Both SIPM and NAR increased (p<0.05) when the thawing extender was supplemented with 12.5, 25 and 50mg CLC/500x10(6) sperm. Nevertheless, motility decreased (p<0.05) when the concentration of CLC exceeded 12.5mg CLC/500x10(6) sperm. In conclusion, our results suggest that the supplementation of thawing extenders with CLC improves sperm viability and reduces acrosome damage after freezing/thawing.