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dc.contributor.authorSalvador, I.
dc.contributor.authorYaniz, J. L.
dc.contributor.authorViudes-De-Castro, María P.
dc.contributor.authorGómez, Ernesto A.
dc.contributor.authorSilvestre, Miguel A.
dc.date.accessioned2017-06-01T10:10:13Z
dc.date.available2017-06-01T10:10:13Z
dc.date.issued2006
dc.identifier.citationSalvador, I., Yaniz, J., Viudes-de-Castro, M.P., Gomez, E.A., Silvestre, M.A. (2006). Effect of solid storage on caprine semen conservation at 5 degrees C. Theriogenology, 66(4), 974-981.
dc.identifier.issn0093-691X
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4475
dc.description.abstractIn this work, we investigated the effect of storage in solid-phase extender on buck semen conserved at 5 degrees C. Furthermore, we studied the effect of addition of cysteine to the extender and the washing of seminal plasma on sperm survival. In Experiment 1, milk-based extender (M) was used as a control to study the effect of solid media storage (G) and cysteine supplementation (C), and the combination of both (GC), on in vitro sperm survival of washed and non-washed semen, conserved up to 72 h at 5 degrees C. Motility, acrosome integrity (NAR) and hypo-osmotic swelling tests (HOST) were evaluated to assess in vitro sperm survival. In Experiment 2, an artificial insemination (AI) field trial was performed to compare G versus M. Solid media (G) maintained motility of spermatozoa during storage higher than any other extender (67% G versus 62% GC; 61% M and 59% C; P 0.05). No improvement in sperm viability was obtained by addition of cysteine to the media. Washing of semen improved motility (65% versus 60%; P 0.05). No improvement in sperm viability was obtained by addition of cysteine to the media. Washing of semen improved motility (65% versus 60%; P 0.05). No improvement in sperm viability was obtained by addition of cysteine to the media. Washing of semen improved motility (65% versus 60%; P 0.05). In conclusion, washing of semen and dilution in gelatin- supplemented milk extender (solid storage) appears to be a successful method for goat semen storage at 5 degrees C. (c) 2006 Elsevier Inc. All rights reserved.
dc.language.isoen
dc.titleEffect of solid storage on caprine semen conservation at 5 degrees C
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormSEP 1 2006
dc.entidadIVIACentro de Tecnología Animal
dc.identifier.doi10.1016/j.theriogenology.2006.02.042
dc.journal.abbreviatedTitleTheriogenology
dc.journal.issueNumber4
dc.journal.titleTheriogenology
dc.journal.volumeNumber66
dc.page.final981
dc.page.initial974
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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