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dc.contributor.authorSalvador, I.
dc.contributor.authorCebrián-Serrano, Alberto
dc.contributor.authorSalamone, D.
dc.contributor.authorSilvestre, Miguel A.
dc.date.accessioned2017-06-01T10:10:13Z
dc.date.available2017-06-01T10:10:13Z
dc.date.issued2011
dc.identifier.citationSalvador, I., Cebrian-Serrano, A., Salamone, D., Silvestre, M.A. (2011). Effect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine. Spanish Journal of Agricultural Research, 9(3), 744-752.
dc.identifier.issn1695-971X
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4471
dc.description.abstractThe aim of this study was to identify the in vitro development stage at which the culture of a single or low number (n = 5 or 10) of oocytes/embryos could impair development in comparison with culture in group (n = 50). In the Experiment 1, it was confirmed that single in vitro embryo production yielded lower cleavage and blastocyst rates than in group (49.4 vs. 83.0%; 0% vs. 37.8%, respectively; p < 0.05). In Experiment 2 and 3, it was observed no effect on embryo development of culturing single or low number of oocytes during maturation and fertilization, respectively. In Experiment 4, it was observed a detrimental effect on blastocyst rate when cultured single or low number of embryos during post-fertilization in vitro culture (2.9; 10.2-10.8; 33.2% in single, low number of embryos (5-10), and control-grouped, respectively; p < 0.05). In Experiment 5, it was observed that the last part of the culture period (day 3 onwards) seemed to be more affected by the low number of embryos placed in culture. In conclusion, post-fertilization culture, especially on days 3 to 7 after fertilization, seems to be the most important stage for embryo development on single and/or low number (5-10) of embryos culture.
dc.language.isoen
dc.titleEffect of number of oocytes and embryos on in vitro oocyte maturation, fertilization and embryo development in bovine
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormSEP 2011
dc.entidadIVIACentro de Tecnología Animal
dc.identifier.doi10.5424/sjar/20110903-374-10
dc.journal.issueNumber3
dc.journal.titleSpanish Journal of Agricultural Research
dc.journal.volumeNumber9
dc.page.final752
dc.page.initial744
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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