A Simple Imprint-Hybridization Method for Detection of Viroids

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URI
http://hdl.handle.net/20.500.11939/4442
DOI
10.1016/0166-0934(95)00043-T
Derechos de acceso
openAccess
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Author
Romerodurban, J.; Cambra, Mariano; Durán-Vila, Núria
Date
1995
Cita bibliográfica
Romerodurban, J., Cambra, M., Duranvila, N. (1995). A Simple Imprint-Hybridization Method for Detection of Viroids. Journal of virological methods, 55(1), 37-47.
Abstract
An imprint-hybridization method has been designed to simplify the processing of samples during routine viroid indexing. The method requires minimal sample manipulation and has been evaluated for detection of viroids in 11 viroid-host combinations including 4 viroids (CEVd, CSVd, HSVd, ASBVd) and 7 hosts (chrysanthemum, citron, cucumber, Gynura, tomato, peach and avocado). The method is fast and sensitive, and provides additional information on the sites of viroid accumulation.
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