A Simple Imprint-Hybridization Method for Detection of Viroids
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Cita bibliográficaRomerodurban, J., Cambra, M., Duranvila, N. (1995). A Simple Imprint-Hybridization Method for Detection of Viroids. Journal of virological methods, 55(1), 37-47.
An imprint-hybridization method has been designed to simplify the processing of samples during routine viroid indexing. The method requires minimal sample manipulation and has been evaluated for detection of viroids in 11 viroid-host combinations including 4 viroids (CEVd, CSVd, HSVd, ASBVd) and 7 hosts (chrysanthemum, citron, cucumber, Gynura, tomato, peach and avocado). The method is fast and sensitive, and provides additional information on the sites of viroid accumulation.