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dc.contributor.authorQuiles-Puchalt, Nuria
dc.contributor.authorMartinez-Rubio, Roser
dc.contributor.authorRam, Geeta
dc.contributor.authorLasa, Inigo
dc.contributor.authorPenadés, José R.
dc.date.accessioned2017-06-01T10:10:00Z
dc.date.available2017-06-01T10:10:00Z
dc.date.issued2014
dc.identifier.citationQuiles-Puchalt, N., Martinez-Rubio, Roser, Ram, Geeta, Lasa, Inigo, Penades, J.R. (2014). Unravelling bacteriophage phi 11 requirements for packaging and transfer of mobile genetic elements in Staphylococcus aureus. Molecular microbiology, 91(3), 423-437.
dc.identifier.issn0950-382X
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4390
dc.description.abstractBacteriophages play a major role in spreading mobile genetic elements (MGEs)-encoded genes among bacterial populations. In spite of this, the molecular requirements for building phage transducing particles have not been completely deciphered. Here, we systematically inactivated each ORF from the packaging and lysis modules of the staphylococcal phage phi 11, used as a model for the Siphoviridae phages infecting Gram-positive bacteria, and determined their functional role in transferring different MGEs including plasmids, staphylococcal pathogenicity islands (SaPIs) and the phage itself. In a previous report, we identified seven of these ORFs as being required for the production of functional phage or SaPI particles. In this report, we have completed the mutational analysis and have identified and characterized 15 additional phage-encoded proteins required for the production of mature phage, SaPI, or transducing particles. Apart from these, we have not yet ascertained any specific function for the six remaining phi 11 genes, though they are highly conserved among the staphylococcal bacteriophages. To the best of our knowledge, this study represents the first systematic deletion analysis of all the ORFs comprising the morphogenetic and lysis modules of a phage, clearly defining the molecular requirements involved in phage-mediated MGEs transfer.
dc.language.isoen
dc.titleUnravelling bacteriophage phi 11 requirements for packaging and transfer of mobile genetic elements in Staphylococcus aureus
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormFEB 2014
dc.entidadIVIACentro de Tecnología Animal
dc.identifier.doi10.1111/mmi.12445
dc.journal.abbreviatedTitleMol.Microbiol.
dc.journal.issueNumber3
dc.journal.titleMolecular microbiology
dc.journal.volumeNumber91
dc.page.final437
dc.page.initial423
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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