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dc.contributor.authorQuiles-Puchalt, Nuria
dc.contributor.authorAngeles Tormo-Mas, Maria
dc.contributor.authorCampoy, Susana
dc.contributor.authorToledo-Arana, Alejandro
dc.contributor.authorMonedero, Vicente
dc.contributor.authorLasa, Inigo
dc.contributor.authorNovick, Richard P.
dc.contributor.authorChristie, Gail E.
dc.contributor.authorPenadés, José R.
dc.date.accessioned2017-06-01T10:10:00Z
dc.date.available2017-06-01T10:10:00Z
dc.date.issued2013
dc.identifier.citationQuiles-Puchalt, N., Angeles Tormo-Mas, M., Campoy, S., Toledo-Arana, A., Monedero, Vicente, Lasa, Inigo, Novick, Richard P., Christie, Gail E., Penades, J.R. (2013). A super-family of transcriptional activators regulates bacteriophage packaging and lysis in Gram-positive bacteria. Nucleic acids research, 41(15), 7260-7275.
dc.identifier.issn0305-1048
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4389
dc.description.abstractThe propagation of bacteriophages and other mobile genetic elements requires exploitation of the phage mechanisms involved in virion assembly and DNA packaging. Here, we identified and characterized four different families of phage-encoded proteins that function as activators required for transcription of the late operons (morphogenetic and lysis genes) in a large group of phages infecting Gram-positive bacteria. These regulators constitute a super-family of proteins, here named late transcriptional regulators (Ltr), which share common structural, biochemical and functional characteristics and are unique to this group of phages. They are all small basic proteins, encoded by genes present at the end of the early gene cluster in their respective phage genomes and expressed under cI repressor control. To control expression of the late operon, the Ltr proteins bind to a DNA repeat region situated upstream of the terS gene, activating its transcription. This involves the C-terminal part of the Ltr proteins, which control specificity for the DNA repeat region. Finally, we show that the Ltr proteins are the only phage-encoded proteins required for the activation of the packaging and lysis modules. In summary, we provide evidence that phage packaging and lysis is a conserved mechanism in Siphoviridae infecting a wide variety of Gram-positive bacteria.
dc.language.isoen
dc.titleA super-family of transcriptional activators regulates bacteriophage packaging and lysis in Gram-positive bacteriaen
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormAUG 2013
dc.entidadIVIACentro de Tecnología Animal
dc.identifier.doi10.1093/nar/gkt508
dc.journal.abbreviatedTitleNucleic Acids Res.
dc.journal.issueNumber15
dc.journal.titleNucleic acids research
dc.journal.volumeNumber41
dc.page.final7275
dc.page.initial7260
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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