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dc.contributor.authorPerez, RM
dc.contributor.authorMas, O.
dc.contributor.authorNavarro, Luis
dc.contributor.authorDurán-Vila, Núria
dc.date.accessioned2017-06-01T10:09:55Z
dc.date.available2017-06-01T10:09:55Z
dc.date.issued1999
dc.identifier.citationPerez, RM, Mas, O., Navarro, L., Duran-Vila, N. (1999). Production and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids. Plant Cell Tissue and Organ Culture, 55(1), 71-74.
dc.identifier.issn0167-6857
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4354
dc.description.abstractAssays were performed to obtain embryogenic callus lines from nine mandarin and mandarin hybrid cultivars by in vitro culture of ovules collected from immature fruits six weeks after anthesis. All cultivars produced embryos and loose friable callus. The small proliferations of nucellar callus from cultured ovules were suitable to recover embryogenic callus lines by periodical subculturing to fresh medium. The embryogenic callus cultures were subjected to cryoprotection with 10% (v/v) (DMSO), freezing by slow cooling, storage in liquid nitrogen and thawing by fast warming. Whole plants from these cryopreserved cultures were recovered through embryogenesis.
dc.language.isoen
dc.titleProduction and cryoconservation of embryogenic cultures of mandarin and mandarin hybrids
dc.typearticle
dc.identifier.doi10.1023/A:1026433405701
dc.journal.issueNumber1
dc.journal.titlePlant Cell Tissue and Organ Culture
dc.journal.volumeNumber55
dc.page.final74
dc.page.initial71
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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