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dc.contributor.authorPérez, R. M.
dc.contributor.authorGaliana, AM
dc.contributor.authorNavarro, Luis
dc.contributor.authorDurán-Vila, Núria
dc.date.accessioned2017-06-01T10:09:55Z
dc.date.available2017-06-01T10:09:55Z
dc.date.issued1998
dc.identifier.citationPerez, RM, Galiana, AM, Navarro, L., Duran-Vila, N. (1998). Embryogenesis in vitro of several Citrus species and cultivars. Journal of Horticultural Science & Biotechnology, 73(6), 796-802.
dc.identifier.issn0022-1589
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4353
dc.description.abstractA number of experiments were performed to obtain somatic embryos and embryogenie cell lines from several species and cultivars of citrus by in vitro ovule culture. The source of ovules (pre-anthesis and post-anthesis), incubation conditions (light and darkness) and several modifications of the culture medium were evaluated. All the species and cultivars assayed produced somatic embryos and “pseudobulbi”, but production of friable embryogenie callus was species dependent. The small proliferation of callus and globular bodies recovered from the primary cultures were suitable for recovering embryogenie callus lines by periodical subculturing into fresh medium. After several monthly subcultures discarding embryos and “pseudobulbi”, cell lines virtually devoid of organized structures were obtained from three cultivars of sweet orange, sour orange and Cleopatra mandarin. In all these species, embryogenesis was restored spontaneously and/or by substitution of sucrose by maltose or lactose in the culture medium. The embryos germinated and produced phenotypically normal plants.
dc.language.isoen
dc.titleEmbryogenesis in vitro of several Citrus species and cultivars
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormNOV
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1080/14620316.1998.11511050
dc.journal.issueNumber6
dc.journal.titleJournal of Horticultural Science & Biotechnology
dc.journal.volumeNumber73
dc.page.final802
dc.page.initial796
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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