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dc.contributor.authorAramburu, J.
dc.contributor.authorNavascastillo, J.
dc.contributor.authorMoreno, Pedro
dc.contributor.authorCambra, Mariano
dc.date.accessioned2017-06-01T10:09:53Z
dc.date.available2017-06-01T10:09:53Z
dc.date.issued1991
dc.identifier.citationAramburu, J., Navascastillo, J., Moreno, P., Cambra, M. (1991). Detection of Double-Stranded-Rna by Elisa and Dot Immunobinding Assay using an Antiserum to Synthetic Polynucleotides. Journal of virological methods, 33(1-2), 1-11.
dc.identifier.issn0166-0934
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4339
dc.description.abstractAn antiserum against polyinosinic-polycytidylic acid (In-Cn) was used to detect double-stranded RNA (dsRNA) by several serological techniques. DsRNA was readily detected by indirect ELISA (ELISA-I) and dot immunobinding assay (DIA). Addition of the antigen to poly-L-lysine-precoated plates and blocking with uncreamed milk powder allowed detection levels of 100 pg.ml-1 In-Cn by ELISA-I. Concentrations as low as 1 ng.ml-1 were detected by DIA using polyvinyliden difluoride (PVDF) membranes. Detection capacity with nitrocellulose membranes was 1000 times lower than with PVDF. ELISA-I and DIA enabled detection of dsRNA in enriched fractions from cucumber mosaic virus (CMV)- and citrus tristeza virus (CTV)-infected plants and from virus-infected Penicillium chrysogenum mycelium. These techniques showed similar or higher sensitivity for detection of dsRNA than separation by polyacrylamide gel electrophoresis and silver staining.
dc.language.isoen
dc.titleDetection of Double-Stranded-Rna by Elisa and Dot Immunobinding Assay using an Antiserum to Synthetic Polynucleotides
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias, Carretera CV-315, Km. 10,7 - 46113 Moncada (València)
dc.date.issuedFreeFormJUN 1991
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1016/0166-0934(91)90002-H
dc.journal.abbreviatedTitleJ.Virol.Methods
dc.journal.issueNumber1-2
dc.journal.titleJournal of virological methods
dc.journal.volumeNumber33
dc.page.final11
dc.page.initial1
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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