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dc.contributor.authorPena, L.
dc.contributor.authorCervera, M.
dc.contributor.authorJuarez, J.
dc.contributor.authorNavarro, A.
dc.contributor.authorPina, JA
dc.contributor.authorNavarro, Luis
dc.date.accessioned2017-06-01T10:09:52Z
dc.date.available2017-06-01T10:09:52Z
dc.date.issued1997
dc.identifier.citationPena, L., Cervera, M., Juarez, J., Navarro, A., Pina, JA, Navarro, L. (1997). Genetic transformation of lime (Citrus aurantifolia Swing): factors affecting transformation and regeneration. Plant Cell Reports, 16(11), 731-737.
dc.identifier.issn0721-7714
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4334
dc.description.abstractWe have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. OsbeckxPoncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We now report on the optimization of the cocultivation, regeneration and selection conditions for efficient and reliable production of transgenic lime (C. aurantifolia Swing.) plants. Improved transformation frequencies were obtained by co-cultivating the explants with Agrobacterium on feeder plates. Optimum regeneration of transgenic shoots was obtained by exposing the explants to darkness for 2 weeks and by using kanamycin at 100 mg/l as selective agent. Attempts to use geneticin as selection antibiotic were not successful. Shoot tip grafting of regenerated shoots on Troyer citrange seedlings resulted in 100% successful production of transgenic plants. The presence and expression of the transferred genes in the regenerated plants was verified by beta-glucuronidase histochemical and fluorimetric assays, neomycin phosphotransferase ELISA assays, PCR and Southern analyses.
dc.language.isoen
dc.titleGenetic transformation of lime (Citrus aurantifolia Swing): factors affecting transformation and regeneration
dc.typearticle
dc.date.issuedFreeFormSEP
dc.journal.abbreviatedTitlePlant Cell Rep.
dc.journal.issueNumber11
dc.journal.titlePlant Cell Reports
dc.journal.volumeNumber16
dc.page.final737
dc.page.initial731
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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