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dc.contributor.authorPena, Eduardo Jose
dc.contributor.authorFerriol, Inmaculada
dc.contributor.authorSambade, Adrian
dc.contributor.authorBuschmann, Henrik
dc.contributor.authorNiehl, Annette
dc.contributor.authorElena, Santiago F.
dc.contributor.authorRubio, Luis
dc.contributor.authorHeinlein, Manfred
dc.date.accessioned2017-06-01T10:09:51Z
dc.date.available2017-06-01T10:09:51Z
dc.date.issued2014
dc.identifier.citationPena, E. J., Ferriol, I., Sambade, Adrian, Buschmann, Henrik, Niehl, Annette, E., Santiago F., Rubio, L., Heinlein, Manfred (2014). Experimental Virus Evolution Reveals a Role of Plant Microtubule Dynamics and TORTIFOLIA1/SPIRAL2 in RNA Trafficking. Plos One, 9(8), e105364-e105364.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4329
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4329
dc.description.abstractThe cytoskeleton is a dynamic network composed of filamentous polymers and regulatory proteins that provide a flexible structural scaffold to the cell and plays a fundamental role in developmental processes. Mutations that alter the spatial orientation of the cortical microtubule (MT) array of plants are known to cause important changes in the pattern of cell wall synthesis and developmental phenotypes; however, the consequences of such alterations on other MT-network-associated functions in the cytoplasm are not known. In vivo observations suggested a role of cortical MTs in the formation and movement of Tobacco mosaic virus (TMV) RNA complexes along the endoplasmic reticulum (ER). Thus, to probe the significance of dynamic MT behavior in the coordination of MT-network-associated functions related to TMV infection and, thus, in the formation and transport of RNA complexes in the cytoplasm, we performed an evolution experiment with TMV in Arabidopsis thaliana tor1/spr2 and tor2 mutants with specific defects in MT dynamics and asked whether TMV is sensitive to these changes. We show that the altered cytoskeleton induced genetic changes in TMV that were correlated with efficient spread of infection in the mutant hosts. These observations demonstrate a role of dynamic MT rearrangements and of the MT-associated protein TORTIFOLIA1/SPIRAL2 in cellular functions related to virus spread and indicate that MT dynamics and MT-associated proteins represent constraints for virus evolution and adaptation. The results highlight the importance of the dynamic plasticity of the MT network in directing cytoplasmic functions in macromolecular assembly and trafficking and illustrate the value of experimental virus evolution for addressing the cellular functions of dynamic, long-range order systems in multicellular organisms.
dc.language.isoen
dc.titleExperimental Virus Evolution Reveals a Role of Plant Microtubule Dynamics and TORTIFOLIA1/SPIRAL2 in RNA Trafficking
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormAUG 18 2014
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1371/journal.pone.0105364
dc.journal.issueNumber8
dc.journal.titlePlos One
dc.journal.volumeNumber9
dc.page.finale105364
dc.page.initiale105364
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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