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dc.contributor.authorPeiro, Ana
dc.contributor.authorCanizares, M. C.
dc.contributor.authorRubio, Luis
dc.contributor.authorLopez, Carmelo
dc.contributor.authorMoriones, Enrique
dc.contributor.authorAramburu, Jose
dc.contributor.authorSanchez-Navarro, Jesus
dc.date.accessioned2017-06-01T10:09:51Z
dc.date.available2017-06-01T10:09:51Z
dc.date.issued2014
dc.identifier.citationPeiro, Ana, Canizares, M.C., Rubio, L., Lopez, Carmelo, Moriones, E., Aramburu, J., Sanchez-Navarro, Jesus (2014). The movement protein (NSm), of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance. Molecular Plant Pathology, 15(8), 802-813.
dc.identifier.issn1464-6722
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4324
dc.description.abstractThe avirulence determinant triggering the resistance conferred by the tomato gene Sw-5 against Tomato spotted wilt virus (TSWV) is still unresolved. Sequence comparison showed two substitutions (C118Y and T120N) in the movement protein NSm present only in TSWV resistance-breaking (RB) isolates. In this work, transient expression of NSm of three TSWV isolates [RB1 (T120N), RB2 (C118Y) and non-resistance-breaking (NRB)] in Nicotiana benthamiana expressing Sw-5 showed a hypersensitive response (HR) only with NRB. Exchange of the movement protein of Alfalfa mosaic virus (AMV) with NSm supported cell-to-cell and systemic transport of the chimeric AMV RNAs into N.tabacum with or without Sw-5, except for the constructs with NBR when Sw-5 was expressed, although RB2 showed reduced cell-to-cell transport. Mutational analysis revealed that N120 was sufficient to avoid the HR, but the substitution V130I was required for systemic transport. Finally, co-inoculation of RB and NRB AMV chimeric constructs showed different prevalence of RB or NBR depending on the presence or absence of Sw-5. These results indicate that NSm is the avirulence determinant for Sw-5 resistance, and mutations C118Y and T120N are responsible for resistance breakdown and have a fitness penalty in the context of the heterologous AMV system.
dc.language.isoen
dc.titleThe movement protein (NSm) of Tomato spotted wilt virus is the avirulence determinant in the tomato Sw-5 gene-based resistance
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormOCT 2014
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1111/mpp.12142
dc.journal.issueNumber8
dc.journal.titleMolecular Plant Pathology
dc.journal.volumeNumber15
dc.page.final813
dc.page.initial802
dc.rights.accessRightsopenAccess
dc.source.typeImpreso
dc.type.hasVersionpublishedVersion


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