Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of 'Xanthomonas arboricola' pv. 'pruni' in plant tissue samples
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AutorPalacio-Bielsa, Ana; Lopez-Soriano, Pablo; Buehlmann, Andreas; van Doom, Joop; Pham, Khanh; Cambra, Miguel A.; Berruete, Isabel M.; Pothier, Joel F.; Duffy, Brion; Olmos, Antonio; Lopez, Maria M.
Cita bibliográficaPalacio-Bielsa, Ana, Lopez-Soriano, P., Buehlmann, Andreas, van Doom, Joop, Pham, Khanh, Cambra, M.A., Berruete, I.M., Pothier, J. F., Duffy, Brion, Olmos, A., Lopez, M.M. (2015). Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of 'Xanthomonas arboricola' pv. 'pruni' in plant tissue samples. Journal of microbiological methods, 112, 36-39.
Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods. (C) 2015 Elsevier B.V. All rights reserved.