Evaluation of a real-time PCR and a loop-mediated isothermal amplification for detection of <I>Xanthomonas arboricola</I> pv. <I>pruni</I> in plant tissue samples
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AutorPalacio-Bielsa,Ana; Lopez-Soriano,Pablo; Buehlmann,Andreas; van Doom,Joop; Pham,Khanh; Cambra,Miguel A.; Berruete,Isabel M.; Pothier,Joel F.; Duffy,Brion; Olmos,Antonio; Lopez,Maria M.
Operational capacity of real-time PCR and loop-mediated isothermal amplification (LAMP) diagnostic assays for detection of Xanthomonas arboricola pv. pruni was established in a ring-test involving four laboratories. Symptomatic and healthy almond leaf samples with two methods of sample preparation were analyzed. Kappa coefficient, sensitivity, specificity, likelihood ratios and post-test probability of detection were estimated to manage the risk associated with the use of the two methods. (C) 2015 Elsevier B.V. All rights reserved.