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dc.contributor.authorOlmos, Antonio
dc.contributor.authorCambra, Mariano
dc.contributor.authorDasi, M. A.
dc.contributor.authorCandresse, Thierry
dc.contributor.authorEsteban, Olga
dc.contributor.authorGorris, María T.
dc.contributor.authorAsensio, M.
dc.date.accessioned2017-06-01T10:09:42Z
dc.date.available2017-06-01T10:09:42Z
dc.date.issued1997
dc.identifier.citationOlmos, A., Cambra, M., Dasi, M.A., Candresse, T., Esteban, O., Gorris, M. T., Asensio, M. (1997). Simultaneous detection and typing of plum pox potyvirus (PPV), isolates by heminested-PCR and PCR-ELISA. Journal of virological methods, 68(2), 127-137.
dc.identifier.issn0166-0934
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4259
dc.description.abstractTwo techniques for simultaneous detection and typing of plum pox potyvirus (PPV) isolates belonging to the D or M serotypes, heminested PCR (H-PCR) and PCR-ELISA, have been developed. Ten PPV isolates typed using PPV-D and PPV-M specific monoclonal antibodies by ELISA-DASI were used to validate these two methods. The results obtained show a complete coincidence of the nucleic acid-based techniques with the serological data. When serial dilutions of infected plant extracts were assayed, H-PCR and PCR-ELISA were found to be 100 rimes more sensitive than the more conventional immunocapture-PCR (IC-PCR) assay. Testing of 228 PPV-infected fruit tree samples coming from different hosts and locations indicated that so fat-only PPV type D appears to be present in Spain and in Chile. Coupled with print-capture sample preparation (Olmos et al., Nucl. Acids Res. 24, 2192-2193, 1996) the increased sensitivity provided by heminested-PCR allowed the detection of PPV targets of D and M types, in wingless individuals of the aphid vector Aphis gossypii. (C) 1997 Elsevier Science Ireland Ltd.
dc.language.isoen
dc.titleSimultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISA
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormNOV 1997
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1016/S0166-0934(97)00120-1
dc.journal.abbreviatedTitleJ.Virol.Methods
dc.journal.issueNumber2
dc.journal.titleJournal of virological methods
dc.journal.volumeNumber68
dc.page.final137
dc.page.initial127
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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