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dc.contributor.authorOlmos, Antonio
dc.contributor.authorBertolini, Edson
dc.contributor.authorGil, M.
dc.contributor.authorCambra, Mariano
dc.contributor.editorLlácer, G.
dc.date.accessioned2017-06-01T10:09:42Z
dc.date.available2017-06-01T10:09:42Z
dc.date.issued2004
dc.identifier.citationOlmos, A., Bertolini, E., Gil, M., Cambra, M. (2004). Real-time RT-PCR for quantitative detection of Plum pox virus. Proceedings of the Xixth International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops: Fruit Tree Diseases, (657), 149-153.
dc.identifier.issn0567-7572; 90-6605-148-5
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4257
dc.description.abstractTwo protocols for real-time quantitative detection of Plum pox virus (PPV) and identification of PPV-D and PPV-M isolates were developed using the intercalating dye SYBR-Green I and TaqMan chemistry. SYBR-Green I method using the universal PPV primers, P1 and P2, required for its optimization an accurate analysis of primers concentration to avoid primer-dimer formation. Optimization for TaqMan technology required the design of new primers and internal probes using the Primer Express software and the alignment of available PPV sequences from Genbank, EMBL, and DDBJ databases. Although the two methods were able to detect as low as 4 copies of transcripts, only the TaqMan method maintained this sensitivity when plant material was used. In addition, the use of TaqMan technology succeed in the detection and quantification of PPV targets in single aphids.
dc.language.isoen
dc.titleReal-time RT-PCR for quantitative detection of Plum pox virus
dc.title.alternativeACTA HORTICULTURAE
dc.typeconferenceObject
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias, Carretera CV-315, Km. 10,7 - 46113 Moncada (València)
dc.date.issuedFreeForm2004
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.17660/ActaHortic.2004.657.19
dc.journal.issueNumber657
dc.journal.titleProceedings of the Xixth International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops: Fruit Tree Diseases
dc.page.final153
dc.page.initial149
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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