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dc.contributor.authorOlmos, Antonio
dc.contributor.authorCambra, M.
dc.contributor.authorEsteban, O.
dc.contributor.authorGorris, María T.
dc.contributor.authorTerrada, E.
dc.date.accessioned2017-06-01T10:09:41Z
dc.date.available2017-06-01T10:09:41Z
dc.date.issued1999
dc.identifier.citationOlmos, A., Cambra, M., Esteban, O., Gorris, M. T., Terrada, E. (1999). New device and method for capture, reverse transcription and nested PCR in a single closed-tube. Nucleic acids research, 27(6), 1564-1565.
dc.identifier.issn0305-1048
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4252
dc.description.abstractA device and improved method based on the use of a compartmentalized Eppendorf tube that allows capture, reverse transcription and nested-PCR in a single closed-tube has been developed and patented. The main advantages of the system are the high sensitivity obtained, the simplicity, the low risk of contamination and the easy establishment of adequate conditions for nested-PCR. The method has been successfully applied to the detection and characterization of citrus tristeza closterovirus and plum pox potyvirus isolates in plant tissues and single aphids squashed on paper. This device and methodology could be easily adapted to the detection of other targets.
dc.language.isoen
dc.titleNew device and method for capture, reverse transcription and nested PCR in a single closed-tube
dc.typearticle
dc.date.issuedFreeFormMAR 15 1999
dc.identifier.doi10.1093/nar/27.6.1564
dc.journal.abbreviatedTitleNucleic Acids Res.
dc.journal.issueNumber6
dc.journal.titleNucleic acids research
dc.journal.volumeNumber27
dc.page.final1565
dc.page.initial1564
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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