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dc.contributor.authorOlivares-Fuster, Oscar
dc.contributor.authorPena, Leandro
dc.contributor.authorDurán-Vila, Núria
dc.contributor.authorNavarro, Luis
dc.date.accessioned2017-06-01T10:09:38Z
dc.date.available2017-06-01T10:09:38Z
dc.date.issued2002
dc.identifier.citationOlivares-Fuster, O., Pena, L., Duran-Vila, N., Navarro, L. (2002). Green fluorescent protein as a visual marker in somatic hybridization. Annals of Botany, 89(4), 491-497.
dc.identifier.issn0305-7364
dc.identifier.urihttp://hdl.handle.net/20.500.11939/4229
dc.description.abstractUsing a transgenic citrus plant expressing Green Fluorescent Protein (GFP) as a parent in somatic fusion experiments, we investigated the suitability of GFP as an in vivo marker to follow the processes of protoplast fusion, regeneration and selection of hybrid plants. A high level of GFP expression was detected in transgenic citrus protoplasts, hybrid callus, embryos and plants, It is demonstrated that GFP can be used for the continuous monitoring of the fusion process, localization of hybrid colonies and callus, and selection of somatic hybrid embryos and plants.
dc.language.isoen
dc.titleGreen fluorescent protein as a visual marker in somatic hybridization
dc.typearticle
dc.authorAddressInstituto Valenciano de Investigaciones Agrarias (IVIA), Carretera CV-315, Km. 10’7, 46113 Moncada (Valencia), Españaes
dc.date.issuedFreeFormAPR
dc.entidadIVIACentro de Protección Vegetal y Biotecnología
dc.identifier.doi10.1093/aob/mcf054
dc.journal.issueNumber4
dc.journal.titleAnnals of Botany
dc.journal.volumeNumber89
dc.page.final497
dc.page.initial491
dc.rights.accessRightsopenAccess
dc.source.typeImpreso


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